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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Production of Polyclonal Antibodies for Detection of Papaya ringspot virus (PRSV) in Ivy Gourd (Coccinia grandis (L.)) ผู้เขียน: Sukanya Noochoo, Wanpen sritongchai,  ดร.ศรีเมฆ ชาวโพงพาง, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe coat protein (CP) gene from the Papaya ringspot virus (PRSV) strain Chiang Mai 1 (PRSV-CP) was cloned and expressed in Escherichic coli and purified using nickel-nitrilotriacetic acid agarose resin. The recombinant coat protein was used as an antigen to produce polyclonal antibodies for detection of PRSV in ivy gourd. The antibodies were able to detect the recombinant protein and PRSV-infected plant samples. Specificity of the antibodies to PRSV was compared with indirect enzyme-linked immunosorbent assay (ELISA), dot-blot immunobinding assay (DIBA) and tissue blot immunoassay (TBIA). It was found that the antibodies were highly specific and could be used to detect the presence of virus in different parts of ivy gourd. The antibodies were used to detect PRSV infection in ivy gourd using indirect ELISA and DIBA on 211 samples collected from papaya orchards. It was shown that 107 samples (50.71%) were infected by PRSV. In this study, results from germination testing on 195 seedlings from the infected ivy gourd seeds implied that PRSV was not seed transmitted. Furthermore, the results from TIBA revealed that PRSV spread to all parts of the ivy gourd (leaf, flower, flower stalk, stem and root, as well as tendril) even though some of the infected parts did not show any viral symptoms. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Proteomics of Papaya ringspot virus-Infected Papaya Leaves) ผู้เขียน:Wanwisa Siriwan, Sittiruk Roytrakul, Motoyuki Shimizu, Naoki Takaya, ดร.ศรีเมฆ ชาวโพงพาง, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe Papaya ringspot virus (PRSV) causes severe economic losses in both papaya and cucurbits throughout the tropical and subtropical regions. An understanding of the interaction between the papaya plant and PRSV can help to improve papaya production. The protein profi les of virus-infected and healthy papaya leaves were compared by two-dimensional polyacrylamide gel electrophoresis. Among the observed 490 protein spots, 227 were identifi ed using matrix-assisted laser desorption/ionizationtime of fl ight mass spectrometry and liquid chromatography-mass spectrometry. Forty-three proteins were found to be similar to those identifi ed in the Papaya EST and NCBI nr databases. They play roles in the areas of: photosynthesis (14%), photorespiration (5%), metabolism (20%), gene and biosynthesis (10%), defence related (7%), stress response (5%), signal transduction (10%), and unknown processes (29%). Spot intensity and transcription levels determined by real-time polymerase chain reaction showed ribulose-1, 5-bisphosphate carboxylase, Rieske protein ubiquinol cytochome C and chlorophyll A/B binding were down-regulated in infected plants. On the other hand, ubiquitin-like modifi ers, vascular processing enzyme and germin-like protein were up-regulated in infected plants at transcription and translation levels. The results showed the novel virus-responding mechanism of the papaya plant that might be essential for developing viral-tolerant papaya in the agricultural industries. |
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ที่มา:วารสารวิทยาศาสตร์เกษตรหัวเรื่อง:ผลกระทบต่อกลุ่มประชากรแบคทีเรียในดินบริเวณรอบรากมะละกอดัดแปลงพันธุกรรมที่ต้านทานต่อไวรัสใบด่างจุดวงแหวนมะละกอ |
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ที่มา:วารสารวิทยาศาสตร์เกษตรหัวเรื่อง:การตรวจสอบการผลิตโปรตีนชนิดใหม่ของมะละกอแขกนวลดัดแปลงพันธุกรรมที่ต้านทานไวรัสใบด่างวงแหวน |
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หัวเรื่อง:การทดสอบประสิทธิภาพของสาร EM ในการป้องกันกำจัดโรคพืช ผู้เขียน:ดร.นิพนธ์ ทวีชัย, ศาสตราจารย์ , Nuttima Boonwatana, Thanawatt kamheangridthirong, ดร.อำไพวรรณ ภราดร์นุวัฒน์, รองศาสตราจารย์ , Preecha Surin, ดร.วิชัย โฆสิตรัตน, รองศาสตราจารย์ , sunetra Phawichit สื่อสิ่งพิมพ์:pdf AbstractEfficacy of Effective Microorganisms (EM) for production of SUTOJU as pesticides to control some important of vegetable and fruit crops was preliminary evaluated under both laboratory and greenhouse at Kasetsart University and Department of Agriculture. In laboratory test, EM or SUTOJU was no effective for growth inhibition of phytopathogenic bacteria and fungi as compared with either antibiotics or fungicides. However, there were some inhibition of bacterial and fungal pathogens in the treatment containing acetic acid which was the ingredient of SUTOJU. The bacteria were comprised of twelve strains of Xanthomonas campestris (X.c.) pv. Citri, and each strain of X.x. pv. campestris, Erwinia carotovora subsp. carotovora and Pseudomonas solanacearum whereas the fungas pathogens were included Colletotrichum capsici, C. gloeosporioides, sclerotium rolfsii. Phytophthora parasitica and Fusarium moniliforme. For the greenhouse experiments, bacterial canker of lime, Phytophthora root rot of citrus, Sclerotium rot of tomato and Papaya ringspot disease were evaluated for efficacy of EM, SUTOJU and SUTOJU ingredient comparing with fungicides and water as control. All EM and SUTOJU treatments were no effective to control the diseases and no significant different from water control whereas fungicide treatments provided more effective control of bacterial and fungal diseases. These results indicated that EM or SUTOJU was no effectiveness to control either bacterial, fungal and viral diseases of crop plants. |